INSERM - UMR1231 - Centre de recherche - Equipe NUTOX : N. Khan

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Préparation d'un gel de protéine, dépôt des échantillons avant électrophorèse.

Coloration au bleu de Coomassie de protéines recombinantes après électrophorèse.

Immunomarquage de protéines après électrophorèse.

Observation microscopique de cellules en culture.

Plaques et boites de culture cellulaire.

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Équipe NUTOX : N. Khan

Lipides, Nutrition, Maladies Rares

Équipes émergentes

Équipe associée

A. Bettaieb

2005-2008

Gaillard D., Passilly-Degrace P., Besnard P. Molecular mechanisms of fat preference and overeating. Ann N Y Acad Sci. 2008 Aug;1141:163-75.

Med Sci

El-Yassimi A, Hichami A, Besnard P, Khan NA. Linoleic acid induces calcium signaling, Src kinase phosphorylation, and neurotransmitter release in mouse CD36-positive gustatory cells. J Biol Chem. 2008 May 9;283(19):12949-59. Epub 2008 Mar 5.
Gaillard D, Laugerette F, Darcel N, El-Yassimi A, Passilly-Degrace P, Hichami A, Akhtar Khan N, Montmayeur JP, Besnard P. The gustatory pathway is involved in CD36-mediated orosensory perception of long-chain fatty acids in the mouse. FASEB J. 2008 Jan 15; [Epub ahead of print].
Laigneau JP, Devaud H, Petit V, Brousse N, Bouvier R, Ferkdadji L, Besmond C, Aggerbeck LP, Bado A, Samson-Bouma ME. Decreased expression, of Intestinal I- and L-FABP levels in rare human genetic malabsorption syndromes. Histochem Cell Biol. 2007 Aug;128(2):115-23. Epub 2007 Jun 29.
Laugerette F, Gaillard D, Passilly-Degrace P, Niot I, Besnard P. Do we taste fat? Biochimie. 2007 Feb;89(2):265-9. Epub 2006 Nov 10. Review.
Petit V, Arnould L, Martin P, Monnot MC, Pineau T, Besnard P, Niot I. Chronic high-fat diet affects intestinal fat absorption and postprandial triglyceride levels in the mouse. J Lipid Res. 2007 Feb;48(2):278-87. Epub 2006 Nov 18.
Poirier H, Shapiro JS, Kim RJ, Lazar MA. Nutritional supplementation with trans-10, cis-12-conjugated linoleic acid induces inflammation of white adipose tissue. Diabetes. 2006 Jun;55(6):1634-41.
Laugerette F, Passilly-Degrace P, Patris B, Niot I, Montmayeur JP, Besnard P. [CD36, a major landmark on the trail of the taste of fat]. Med Sci (Paris). 2006 Apr;22(4):357-9. French. No abstract available.
Thomas C, Landrier JF, Gaillard D, Grober J, Monnot MC, Athias A, Besnard P. Cholesterol dependent downregulation of mouse and human apical sodium dependent bile acid transporter (ASBT) gene expression: molecular mechanism and physiological consequences. Gut. 2006 Sep;55(9):1321-31. Epub 2006 Feb 16.
Laugerette F, Passilly-Degrace P, Patris B, Niot I, Febbraio M, Montmayeur JP, Besnard P. CD36 involvement in orosensory detection of dietary lipids, spontaneous fat preference, and digestive secretions. J Clin Invest. 2005 Nov;115(11):3177-84.
Landrier JF, Thomas C, Grober J, Zaghini I, Petit V, Poirier H, Niot I, Besnard P. The gene encoding the human ileal bile acid-binding protein (I-BABP) is regulated by peroxisome proliferator-activated receptors. Biochim Biophys Acta. 2005 Jun 15;1735(1):41-9.
Poirier H, Rouault C, Clément L, Niot I, Monnot MC, Guerre-Millo M, Besnard P. Hyperinsulinaemia triggered by dietary conjugated linoleic acid is associated with a decrease in leptin and adiponectin plasma levels and pancreatic beta cell hyperplasia in the mouse. Diabetologia. 2005 Jun;48(6):1059-65. Epub 2005 May 3.
Poirier H, Niot I, Clément L, Guerre-Millo M, Besnard P. Development of conjugated linoleic acid (CLA)-mediated lipoatrophic syndrome in the mouse. Biochimie. 2005 Jan;87(1):73-9. Review.

2000-2004

Drozdowski L, Clement L, Keelan M, Niot I, Clandinin MT, Agellon L, Wild G, Besnard P, Thomson AB. Dietary lipids modify intestinal lipid-binding protein RNA abundance in diabetic and control rats. Digestion. 2004;70(3):192-8. Epub 2004 Dec 17.
Landrier JF, Thomas C, Grober J, Duez H, Percevault F, Souidi M, Linard C, Staels B, Besnard P. Statin induction of liver fatty acid-binding protein (L-FABP) gene expression is peroxisome proliferator-activated receptor-alpha-dependent. J Biol Chem. 2004 Oct 29;279(44):45512-8. Epub 2004 Aug 27.
Besnard P, Landrier JF, Grober J, Niot I. [Is the ileal bile acid-binding protein (I-BABP) gene involved in cholesterol homeostasis?] Med Sci (Paris). 2004 Jan;20(1):73-7. Review. French
Landrier JF, Grober J, Demydchuk J, Besnard P. FXRE can function as an LXRE in the promoter of human ileal bile acid-binding protein (I-BABP) gene.FEBS Lett. 2003 Oct 23;553(3):299-303.
Grober J, Lucas S, Sörhede-Winzell M, Zaghini I, Mairal A, Contreras JA, Besnard P, Holm C, Langin D. Hormone-sensitive lipase is a cholesterol esterase of the intestinal mucosa. J Biol Chem. 2003 Feb 21;278(8):6510-5. Epub 2002 Dec 13
Landrier JF, Grober J, Zaghini I, Besnard P. Regulation of the ileal bile acid-binding protein gene: an approach to determine its physiological function(s). Mol Cell Biochem. 2002 Oct;239(1-2):149-55. Review.
Besnard P, Niot I, Poirier H, Clément L, Bernard A. New insights into the fatty acid-binding protein (FABP) family in the small intestine. Mol Cell Biochem. 2002 Oct;239(1-2):139-47. Review.
Clément L, Poirier H, Niot I, Bocher V, Guerre-Millo M, Krief S, Staels B, Besnard P. Dietary trans-10,cis-12 conjugated linoleic acid induces hyperinsulinemia and fatty liver in the mouse. J Lipid Res. 2002 Sep;43(9):1400-9.
Zaghini I, Landrier JF, Grober J, Krief S, Jones SA, Monnot MC, Lefrere I, Watson MA, Collins JL, Fujii H, Besnard P. Sterol regulatory element-binding protein-1c is responsible for cholesterol regulation of ileal bile acid-binding protein gene in vivo. Possible involvement of liver-X-receptor. J Biol Chem. 2002 Jan 11;277(2):1324-31. Epub 2001 Oct 29
Poirier H, Niot I, Monnot MC, Braissant O, Meunier-Durmort C, Costet P, Pineau T, Wahli W, Willson TM, Besnard P. Differential involvement of peroxisome-proliferator-activated receptors alpha and delta in fibrate and fatty-acid-mediated inductions of the gene encoding liver fatty-acid-binding protein in the liver and the small intestine. Biochem J. 2001 Apr 15;355(Pt 2):481-8.

1995-1999

Grober J, Zaghini I, Fujii H, Jones SA, Kliewer SA, Willson TM, Ono T, Besnard P. Identification of a bile acid-responsive element in the human ileal bile acid-binding protein gene. Involvement of the farnesoid X receptor/9-cis-retinoic acid receptor heterodimer. J Biol Chem. 1999 Oct 15;274(42):29749-54.
Foucaud L, Grillasca J, Niot I, Domingo N, Lafont H, Planells R, Besnard P. Output of liver fatty acid-binding protein (L-FABP) in bile. Biochim Biophys Acta. 1999 Jan 4;1436(3):593-9.
Foucaud L, Niot I, Kanda T, Besnard P. Indirect dexamethasone down-regulation of the liver fatty acid-binding protein expression in rat liver. Biochim Biophys Acta. 1998 Mar 30;1391(2):204-12.
Poirier H, Braissant O, Niot I, Wahli W, Besnard P. 9-cis-retinoic acid enhances fatty acid-induced expression of the liver fatty acid-binding protein gene. FEBS Lett. 1997 Aug 4;412(3):480-4.
Poirier H, Niot I, Degrace P, Monnot MC, Bernard A, Besnard P. Fatty acid regulation of fatty acid-binding protein expression in the small intestine. Am J Physiol. 1997 Aug;273(2 Pt 1):G289-95.
Poirier H, Mathieu Y, Besnard P, Bernard A. Intestinal lipid esterification and aging in mice and rats. Comp Biochem Physiol A Physiol. 1997 Mar;116(3):253-60.
Niot I, Poirier H, Besnard P. Regulation of gene expression by fatty acids: special reference to fatty acid-binding protein (FABP). Biochimie. 1997 Feb-Mar;79(2-3):129-33.
Meunier-Durmort C, Poirier H, Niot I, Forest C, Besnard P. Up-regulation of the expression of the gene for liver fatty acid-binding protein by long-chain fatty acids. Biochem J. 1996 Oct 15;319 ( Pt 2):483-7.
Poirier H, Degrace P, Niot I, Bernard A, Besnard P. Localization and regulation of the putative membrane fatty-acid transporter (FAT) in the small intestine. Comparison with fatty acid-binding proteins (FABP). Eur J Biochem. 1996 Jun 1;238(2):368-73.
Kanda T, Niot I, Foucaud L, Fujii H, Bernard A, Ono T, Besnard P. Effect of bile on the intestinal bile-acid binding protein (I-BABP) expression. In vitro and in vivo studies. FEBS Lett. 1996 Apr 15;384(2):131-4.
Mathieu Y, Caselli C, Bernard A, Carlier H. Partition of oleic acid between the lymph and portal blood in rats having a diverted bile-pancreatic duct. Br J Nutr. 1996 Feb;75(2):249-61.
Besnard P, Niot I, Bernard A, Carlier H. Cellular and molecular aspects of fat metabolism in the small intestine. Proc Nutr Soc. 1996 Mar;55(1B):19-37. Review. No abstract available.
Besnard P, Foucaud L, Mallordy A, Berges C, Kaikaus RM, Bernard A, Bass NM, Carlier H. Expression of fatty acid binding protein in the liver during pregnancy and lactation in the rat. Biochim Biophys Acta. 1995 Sep 14;1258(2):153-8.
Mallordy A, Poirier H, Besnard P, Niot I, Carlier H. Evidence for transcriptional induction of the liver fatty-acid-binding-protein gene by bezafibrate in the small intestine. Eur J Biochem. 1995 Feb 1;227(3):801-7.

1990-1994

Mathieu Y, Bernard A, Carlier H. Incorporation of exogenous unsaturated fatty acids in mouse jejunal explants. Ann Nutr Metab. 1994;38(1):48-60.
Besnard P, Mallordy A, Carlier H. Transcriptional induction of the fatty acid binding protein gene in mouse liver by bezafibrate. FEBS Lett. 1993 Jul 26;327(2):219-23.
Mallordy A, Besnard P, Carlier H. Research of an in vitro model to study the expression of fatty acid-binding proteins in the small intestine. Mol Cell Biochem. 1993 Jun 9-23;123(1-2):85-92.
Caselli C, Bernard A, Bezard J, Carlier H. Erucic acid metabolism in rat liver. A combined biochemical and radioautographical study. Arch Int Physiol Biochim Biophys. 1992 Sep-Oct;100(5):309-20.
Pavero C, Bernard A, Carlier H. Administration modalities and intestinal lymph absorption of arachidonic acid in rats. J Nutr. 1992 Aug;122(8):1672-81.
Bernard A, Caselli C, Blond JP, Carlier H. Diet fatty acid composition, age and rat jejunal microvillus enzyme activities. Comp Biochem Physiol Comp Physiol. 1992 Mar;101(3):607-12.
Bernard A, Carlier H. Absorption and intestinal catabolism of fatty acids in the rat: effect of chain length and unsaturation. Exp Physiol. 1991 May;76(3):445-55.
Bernard A, Caselli C, Carlier H. Linoleic acid chyloportal partition and metabolism during its intestinal absorption. Ann Nutr Metab. 1991;35(2):98-110.
Carlier H, Bernard A, Caselli C. Digestion and absorption of polyunsaturated fatty acids. Reprod Nutr Dev. 1991;31(5):475-500. Review.
Besnard P, el M'Selmi A, Jousset U, Collignon H, Garel JM. Effects of 1,25-dihydroxycholecalciferol and calcium on calcitonin mRNA levels in suckling rats. Mol Cell Endocrinol. 1991 Aug;79(1-3):45-52.
Besnard P, Bernard A, Carlier H. [Quantification of mRNA coding for enterocyte fatty acid binding proteins (FABP) in rats: effect of high lipid diet and starvation] C R Acad Sci III. 1991;312(8):407-13. French.
Hussenet MP, Pavero C, Bernard A, Carlier H. Monensin and 14C oleic acid absorption in the rat. Food Addit Contam. 1990;7 Suppl 1:S168-71. No abstract available.
Bernard A, Fleith M, Carlier H. Effect of albumin on oleic acid lymphatic absorption in rats. Comp Biochem Physiol A.

CHAPITRES D'OUVRAGES

Passilly-Degrace P. , Gaillard D., Besnard P. (2008) orosensory perception of dietary lipids in mammals. In “Chemosensory Systems in Mammals, Fishes and Insects”*, S. KORSCHING & W. MEYERHOF (Eds), Springer, germany (in press)
Niot I., Besnard P. (2004) Intestinal uptake and transport of fatty acids. In "Lipobiology", Van der Vusse G.J. (Ed), Elsevier Science, The Netherlands, Adv. Mol. Cell Biol. 33, pp 9-28
Niot I., Besnard P. (2003) Intestinal fat absorption : what role(s) for lipid-binding proteins and peroxisome proliferator-activated receptors ? in "Mammalian lipid-binding proteins : roles in cell homeostasis ", DUTTA-ROY A., SPENER F. (Eds), Viley-VCH Verlag, Germany, pp 359-378.
Niot I., Besnard P. (2004) Intestinal uptake and transport of fatty acids. in “Lipobiology”, VAN DER VUSSE G.J. (Ed), Elsevier Science, the Netherlands Adv. Mol. Cell Biol. 33, pp 9-28.
Niot I., Besnard P. (2003) Intestinal fat absorption : what role(s) for lipid-binding proteins and peroxisome proliferator-activated receptors ? in "Mammalian lipid-binding proteins : roles in cell homeostasis ", DUTTA-ROY A., SPENER F. (Eds), Viley-VCH Verlag, Germany, pp 359-378.

ARTICLES DIDACTIQUES

Khan, N.A., Besnard P. Sensing of lipids via receptor signalling in taste buds. Eur. J. Lipid Sci. Technol. 2008, 110, 379–380
Gaillard D., Martin C., Passilly-Degrace P., Besnard P. Rôle des lipides dans la régulation du comportement alimenatire, Oléagineux, Corps gras, Lipids (OCL) *2008 (in press)
Gaillard D., Passilly-Degrace P., Besnard P. Perception gustative des lipides alimentaires : paradigme et paradoxes, Oléagineux, Corps gras, Lipids (OCL) *2008 (in press)
Besnard P. Le gras est-il une saveur ? Psycho & Cerveau 24 : 44-47 (Revue grand public du groupe « Pour la Science »)
Petit V., Niot I., Poirier H., Besnard P. Absorption intestinale des acides gras : faits et incertitudes. Nutr. Clin. Metab 2007 , 21 : 38-45
Gaillard D., Passilly-Degrace P., Laugerette F., Besnard P. Sur la piste du «Goût du gras » Oléagineux, Corps gras, Lipids (OCL) 2006, 13: 1-6.
Besnard P. Point de vue sur les CLA. La lettre d'information Scientifique CANDIA 2005, n°17.
Poirier H., Niot I., Guerre-Millo M., Besnard P. CLA et gestion du poids: bénéfices escomptés, risques potentiels. Nutrition, Aliments Santé, Aliments Fonctionnels (NAFAS) 2005, 3: 41-45
Besnard P., Poirier H., Niot I., Guerre-Millo M. Les CLA, "nouveaux nutriments" ? Médecine & Nutrition. 2005, 41: 1-6
Besnard P., Poirier H., Niot I., Guerre-Millo M. CLA et syndrome lipoatrophique chez la souris. Oléagineux, Corps gras, Lipides (OCL) 2005, 12: 51-55
Quignard-Boulanger A., Bouthegourd J.C., Yvan-Charvet L., Even P., Besnard P., Martin J.C. Effet anti-obésité des CLA: mythe ou réalité ? Oléagineux, Corps gras, Lipides (OCL) 2005, 12: 45-50
Besnard P., Niot I. (2000) Role of lipid-binding proteins in intestinal absorption of fatty acids. in "Fat digestion and absorption", Christophe A., DE VRIESE S. (Eds), AOCS Press Champain IL 61821-5930 USA, pp 96-118.
Besnard P., Niot I., Poirier H., Bernard A., Caselli C. (1997) Aspects cellulaires et moléculaires de l'absorption intestinale des acides gras. Oléagineux, Corps gras, Lipids (OCL)* *4, 185-191.**
Carlier H., Bernard A., Besnard P., Caselli C. (1992) Les propriétés nutritionnelles des acides gras. In Les cahiers de l’ENSBANA, n° 8, pp 69-91.
Carlier H., Bernard A., Besnard P., Caselli C. (1991) Nature des acides gras et absorption. Rev. Int. Ped., numéro exceptionnel, 12-18.

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